Proteomic

Scientific and Clinical Applications

Protein Discovery
  • Biomarkers
  • Molecular Basis of Disease
  • Molecular Classification
  • Diagnostic, Predictive and Prognostic Cancer Markers
  • Early Detection
  • Activated Kinases
  • Drug Response (ADR, Resistance, Responders, Non-Responders, Hypersensitivity)
  • Outcome Prediction
  • Disease Management
  • Risk Factors
  • Drug Targets
  • Immune Response
  • Autoantibodies Against Disease Proteins
  • Pattern Analysis

Protein Purification

Protein Identification

  • Peptide Mapping
  • Sequencing

Protein-Protein Interactions

  • Epitope Mapping
  • Posttranslational Modifications
  • Phosphorylation
  • Acetylation
  • Glycosylation
  • Methylation
  • Ubiquitination

Protein Quantitation

  • Metabolic Isotope Labeling
  • ICAT (isotope-coded affinity tag)

Tissue Imaging

Assay Development

Services

The Proteomics Core will provide a comprehensive array of services pertaining to the design, performance and analysis of proteomic studies. The core not only offers the ability to implement and validate previously developed proteomic methods to profile proteins in biological fluids and tissues, to quantitate proteins, to identify proteins and posttranslational modifications, and to study protein-protein interactions, but also the capability to modify or develop entirely new assays when warranted.
A consultative service provided by key members of the core will be available to investigators to assess the rationale and feasibility for undertaking specific projects, designing proteomic studies, process and protocol development, proteomic data analysis, and the interpretation of results.

Proteomics Services

  • Relative quantitation by µLC/MS/MS
    • ITRAQ – 8-plex isobaric peptide tagging system that enables you to label all primary amines, regardless of peptide class
  • Protein identification by LC-MALDI and LC/MS/MS
    • coomassie & silver stained gel bands
  • Identification of protein and peptide modifications.
    • phosphorylation sites.
    • protein modifications such as acetylation, methylation, and ubiqitination.
    • coomassie stain only, purified proteins.
  • Protein profiling of complex biological samples, eg. serum, urine, tissue, cell extracts.
    • Profiling by µLC/MS.
    • Multidimensional protein fractionation by µLC

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